Debecker, Damien P.Cannella, DavidOvaert, JustinJustinOvaert2025-05-142025-05-142025-05-142023https://hdl.handle.net/2078.2/33811For more than a decade now, consensus among scientists is that anthropogenic greenhouse gas (GHG) emissions are responsible for the currently observed climate change. Cutting down anthropogenic GHG emissions, including those linked to industrial processes for the production of chemicals is thus essential. In this context, the combination of the low energetic demands and bio-based nature of enzymatic catalysis together with the valorization of GHG such as CO2 and CH4 seems appealing, both from an economic and environmental point of view. Yet, due to a lack of economically viable solutions, the potential of this technology remains unharnessed. Among others, CO2 can be reduced into formic acid, a valuable commodity chemical, by formate dehydrogenases (FDH, EC 1.2.1.2). CH4 on the other hand can be converted into methanol by methane monooxygenase (MMO). However, these redox enzymatic conversions are limited by poor turnover or rely on expensive, unstable cofactors like NADH, or both. In recent years, the combination of photocatalysis and enzymes (= photo-biocatalysis, PBC) has proved effective in overcoming these problems. However, many of the currently developed PBC systems rely on harmful metals and chemicals or still exploit NADH, thereby going against the principles of green chemistry. In this work, we have thus focused on developing PBC systems that adhere as closely as possible to the principles of green chemistry for cofactor-dependent FDHs from Thiobacillus spp. KNK65MA and Mycobacterium vaccae for the conversion of CO2 into formic acid and for soluble MMO (sMMO) from Methylococcus capsulatus for the conversion of CH4 into methanol.BiocatalysisPhoto-biocatalysisGreenhouse gasCarbon dioxydeMethaneFormate dehydrogenaseSoluble methane monooxygenasetext::thesis::master thesisthesis:43332