Hallet, BernardSwinnen, MariusMariusSwinnen2025-05-142025-05-142025-05-142024https://hdl.handle.net/2078.2/36554This master thesis aims to identify potential transposition partners of the bacterial transposon Tn4430. Aligned with the broader goal of comprehending the transposition dynamics of Tn4430 and related Tn3-family transposons, we established a proximity labelling assay in E. coli. This assay aimed to pinpoint host proteins in the vicinity of the transposase of Tn4430. Validating the identified proteins as interactants was achieved through bacterial-two-hybrid assays. This analysis identified multiple potential interactants of TnpA, with several that were previously shown to play a role in the transposition mechanism of several other elements. Further exploration is needed to ascertain the functional roles of these interactions in the transposition mechanism of Tn4430.Tn4430TranspositionProximity labellingTn3-family of transposonsReplication hijackingE. colitext::thesis::master thesisthesis:44184