Dupont-Gillain, Christine C.vander Straeten, AurélienVan de Velde, VictoireVictoireVan de Velde2025-07-022025-07-022025-06-1020252025-06-10https://hdl.handle.net/2078.2/43381According to the World Health Organization, cancer is one of the leading causes of death worldwide. It is estimated that around one in five people will develop the disease at some point in their lives, with around one in nine men and one in twelve women dying from it. Despite advances in conventional treatments such as surgery, chemotherapy, and radiotherapy, many forms of cancer remain difficult to treat effectively. In this context, mRNA-based vaccines have emerged as a promising therapeutic strategy. Initially developed for infectious diseases, these vaccines now offer new perspectives in oncology by enabling targeted immune responses against tumor-specific antigens. This master thesis was conducted as part of an industrial research project focusing on RNA-based technologies. The work contributes to broader efforts to improve understanding of the biological mechanisms involved in delivering nucleic acid-based therapeutics. The objective was to explore cellular uptake pathways relevant to the intracellular delivery of genetic material, with the long-term aim of supporting the development of more efficient delivery systems. A variety of scientific and technical skills were utilized throughout the project, including literature analysis, experimental design, cell culture, and fluorescence-based assays. The experimental work involved a comparative analysis of various nanoparticle formulations, employing well-established cellular and molecular biology techniques. Due to the confidential nature of this project, specific results and proprietary materials are not disclosed in this summary.text::thesis::master thesis