Muccioli, GiulioYagoubi, HafsaHafsaYagoubi2025-05-142025-05-142025-05-142023https://hdl.handle.net/2078.2/31346Recognized as an increasingly concerning health problem in our society, obesity is associated with a systemic low-grade inflammation as well as a local inflammation of the adipose tissue. N-acylethanolamines (NAEs), such as N-arachidonoylethanolamine, N-palmitoylethanolamine and N-oleoylethanolamine, are a family of bioactive lipids involved in several physiological processes such as the control of food intake, fat mass (i.e. adipogenesis) and inflammation. Their levels are regulated by two hydrolyzing enzymes, namely fatty acid amid hydrolase (FAAH) and N-acylethanolamine-hydrolyzing acid amidase (NAAA). Based on the properties of NAEs and previous studies on the inhibition of FAAH and NAAA in the context of inflammation, we aimed to study and to compare the consequences of the inhibition of these enzymes on adipose tissue inflammation and adipogenesis. Hence, during my internship, we assessed the effects of NAAA (i.e. AM11095) and FAAH (i.e. PF-3845) inhibitors on 3T3-L1 cells differentiation into adipocytes using the absorbance of Oil red O as readout. Then, we used human and mice (lean and obese) subcutaneous adipose tissue explants activated or not, ex vivo, with lipopolysaccharides (LPS) and incubated them with the same inhibitors. Their expression and secretion of inflammatory cytokines (e.g. TNF-α, IL-6, MCP-1) were assessed and compared in the different groups. Lastly, data from in vivo experiments were obtained using high-fat diet-induced mice model of obesity. Different types of adipose tissues were recovered and the mean adipocytes size, as well as liver fat content, was measured in the different groups at histological level. To support our previous results, mRNA levels of different cytokines and genes implicated in the inflammation and function of adipose tissue were assessed by RT-qPCR according to what has been found on the literature about NAEs and their receptors roles in adipose tissue.EndocannabinoidsObesitytext::thesis::master thesisthesis:38914