Identification de marqueurs de sénescence cérébrale pour une meilleure compréhension de la maladie d’Alzheimer

Details

Supervisors

Kienlen-Campard, Pascal ; Suelves Caballol, Nuria

Faculty

Faculté de pharmacie et des sciences biomédicales

Degree label

Master [120] en sciences biomédicales, à finalité approfondie

Abstract

enSenescence is a process classically characterized by a permanent cell cycle arrest and other phenotypic alterations. It was mostly studied in proliferative cells. However, recent data suggest that neurons, which are post-mitotic differentiated cells, could also develop a senescence-like phenotype and that senescence could be a key underlying mechanism for age-related neurodegenerative pathologies, including Alzheimer’s disease (AD). The objective of our study is to characterize and to identify markers for cerebral and neuronal senescence by using primary neuronal cultures and cortical tissue from Terc Knock Out mice (TercKO). These mice are a model of accelerated senescence and present systemic senescence at the third generation, due to telomere shortening. The expression of several known markers of replicative senescence (including p21, p16, p19, inflammatory molecules) was evaluated in aging primary cultures of neurons and in WT and TercKO cortical tissue and primary neuronal cultures.