Effect of flavonoid on vertebral bone of osteogenesis imperfecta mice

Details

Supervisors

Behets Wydemans, Catherine ; Chretien, Antoine

Faculty

Faculté des sciences de la motricité

Degree label

Master [120] en sciences de la motricité, orientation générale, à finalité approfondie

Abstract

Background and aims: Osteogenesis imperfecta is a genetic bone disorder due to an abnormality of type I collagen. The aim of the study was to examine effects of a flavonoid (C-substance) on weight, pelvic fractures rate and vertebrae bone parameters in osteogenesis imperfecta mice (oim/oim). Materials and methods: This randomized blinded trial included 64 mice divided into 4 groups: control wildtype (WT) and oim/oim and treated WT and oim/oim. C-substance was administered via drinking water from 5 weeks of age for 12 weeks. The mice were weighed each week during the experiment. Fractures were counted with in vivo NanoSPECT/CT PLUS images. Bone mineral density and cross section area of L2 and L3 vertebrae were assessed ex vivo with pQCT (peripheral Quantitative Computed Tomography). Results: oim/oim mice were significantly lighter than WT throughout the experiment without differences between control and treated groups. Pelvic fracture number was decreased by 59.15% (p = 0.0067) in treated oim/oim at 17 weeks. Oim/oim vertebrae showed significantly lower cross section area, cortical area and bone mineral density than WT ones. C-substance treatment did not impact these parameters in WT and oim/oim mice. Conclusion: 12 weeks of C-substance treatment reduced pelvic fracture number in oim/oim but did not modify weight and pQCT vertebral bone parameters. Further investigations will focus on osteoblast and osteoclast activity by assessing seric markers and histomorphometry.